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磷酸盐缓冲液(PBS)通常在实验室中被作为稀释液、冲洗细胞悬浮液以及用于清洗培养瓶和培养皿,也常作为细胞培养基的添加物。这项研究评估了含有不同浓度的钙离子和镁离子的PBS溶液基质对人外周血液单个核细胞(PB-MCs)趋化因子释放的影响。当PBMCs暴露于含量增加的+/+PBS中时,发现白介素-8也会相应增加。因为细胞外钙离子浓度对细胞迁移有一定影响,趋化因子的分泌表明细胞培养过程中增加钙离子浓度是另一种很重要的调节机理。
Phosphate buffered saline (PBS) is often used as a diluent in the laboratory, rinsed cell suspensions, and used to wash flasks and dishes, often as an addition to cell culture media. This study evaluated the effect of PBS solution matrices containing different concentrations of calcium and magnesium ions on chemokine release of human peripheral blood mononuclear cells (PB-MCs). When PBMCs were exposed to increasing amounts of + / + PBS, interleukin-8 was also found to increase accordingly. Because extracellular calcium concentration has some influence on cell migration, chemokine secretion suggests that increasing calcium concentration during cell culture is another important regulatory mechanism.