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材料和方法作者研究10名29～60岁进展性牙周炎患者。由9名22～29岁牙周组织正常健康人作对照组。根据末梢血中嗜硷粒细胞在体外与自体抗原接触时释放组胺能力,确定机体过敏性致敏作用的存在。为了制备牙龈自体抗原溶液,使用牙周炎患者手术时切除的病变组织。为了进行血中嗜硷粒细胞过敏反应,利用缓冲剂A,它含有25mM羟甲基氨基甲烷[Tpиc(Oкcимe-тил)атидометада],pH7.35±0.05,120mMNaCl,5mMKCl,0.3mg/ml人血清白蛋白。还使用缓冲剂Б,它与缓冲剂A区别在于含0.6mM Ca~(2+)
Materials and Methods The authors studied 10 patients with progressive periodontitis between 29 and 60 years old. By nine 22 to 29-year-old periodontal normal healthy people as a control group. According to the peripheral blood of basophils in vitro and autologous antigen release histamine release ability to determine the presence of allergic sensitization of the body. In order to prepare a solution of gingival self-antigen, diseased tissue excised upon surgery of periodontitis patients is used. For the blood basophil hypersensitivity reaction, buffer A was used which contained 25 mM hydroxymethyl aminomethane [Tpиc (Oкcимe-тил) атидометада], pH 7.35 ± 0.05,120 mM NaCl, 5 mM KCl, 0.3 mg / ml human serum albumin. Buffer Б is also used, which differs from Buffer A in that it contains 0.6 mM Ca2 +