论文部分内容阅读
目的探讨血管细胞粘附分子-1(VCAM-1)修饰的人脐血基质细胞(hUCBSC)对造血干细胞或祖细胞的扩增作用。方法构建 VCAM-1-pcDNA3.1(+)真核表达载体,转染原代培养的人脐血基质细胞,采用半定量逆转录聚合酶链反应(RT-PCR)法和免疫细胞化学技术检测转染前后 hUCBSC及 VCAM-1的表达情况;以 VCAM-1修饰
Objective To investigate the effect of human umbilical cord blood stromal cells (hUCBSC) modified by vascular cell adhesion molecule-1 (VCAM-1) on the proliferation of hematopoietic stem cells or progenitor cells. Methods The eukaryotic expression vector of VCAM-1-pcDNA3.1 (+) was constructed and transfected into human umbilical cord blood stromal cells. The expression of VCAM-1-pcDNA3.1 (+) was detected by semi- quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry The expression of hUCBSC and VCAM-1 before and after transfection; modified with VCAM-1