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目的探讨应用等离子体电解氧化(plasma electrolytic oxidation,PEO)技术处理钛表面后对成骨细胞(人成骨肉瘤Saos-2细胞)早期增殖与分化的影响。方法应用PEO技术在纯钛表面制备多孔氧化钛陶瓷膜(PEO组),并以钛表面机械抛光处理(机械抛光组)及喷砂酸蚀处理(喷砂酸蚀组)作为对照,应用场发射扫描电镜、粗糙度测试仪分析表面形貌。将Saos-2细胞接种于3组样品表面,通过对样品表面细胞形态、细胞增殖及碱性磷酸酶(ALP)活性的检测,分析3组样品对Saos-2早期生物学行为的影响。结果应用PEO法可以在纯钛表面形成多孔结构。Saos-2在3组样品表面的黏附与增殖差异无统计学意义(P>0.05);PEO组的ALP活性高于机械抛光组与喷砂酸蚀组(P<0.05)。结论 PEO处理后的多孔钛表面能促进Saos-2的早期分化功能。
Objective To investigate the effect of plasma electrolytic oxidation (PEO) on the proliferation and differentiation of osteoblasts (human osteosarcoma Saos-2 cells) after titanium surface treatment. Methods Porous titanium oxide ceramic films (PEO) were prepared on the surface of pure titanium by PEO technique. The surface mechanical polishing (mechanical polishing) and sandblasting (sandblasting etched) Scanning electron microscopy, roughness tester analysis of the surface morphology. Saos-2 cells were seeded on the surface of three groups of samples. The effects of three samples on the early biological behavior of Saos-2 were analyzed by measuring the cell morphology, cell proliferation and alkaline phosphatase (ALP) activity on the sample surface. Results PEO method can be used to form a porous structure on the surface of pure titanium. There was no significant difference in the adhesion and proliferation of Saos-2 between three groups of samples (P> 0.05). The ALP activity of PEO group was higher than that of mechanical polishing group and sandblasting acid etching group (P <0.05). Conclusion The surface of porous titanium treated with PEO can promote the early differentiation of Saos-2.