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白细胞介素-1(IL-1)为一种来自单核-巨噬细胞的多肽,能触发许多靶细胞表达各种免疫反应,故这种单核细胞因子在许多免疫炎症性疾患的发病机理中可能起主要的免疫调节作用。已证明IL-1刺激人嗜中性白细胞(Neu)的趋化反应。本文研究IL-1的分泌能力及其激活Neu功能的必要条件。实验用人静脉血经右旋糖酐沉降法、Ficoll—Hypaque 离心分离Neu后者经37℃培养后,离心取上清作髓过氧化物酶(MPO)、溶菌酶和维生素B_(12)结合蛋白(VB_(12)-BP)活性测定。结果观察到IL-1激发经松胞素B(CB)
Interleukin-1 (IL-1), a polypeptide from monocytes-macrophages, triggers the expression of a variety of immune responses in many target cells. Therefore, this monocyte factor is involved in the pathogenesis of many immune-inflammatory disorders May play a major immunomodulatory role. IL-1 has been shown to stimulate the chemotactic response of human neutrophils (Neu). This article studies the secreting capacity of IL-1 and the necessary conditions for its activation of Neu function. In the experiment, human venous blood was separated by dextran sedimentation method and centrifuged with Ficoll-Hypaque. The latter was cultured at 37 ℃ and centrifuged to obtain myeloperoxidase (MPO), lysozyme and vitamin B 12 binding protein (VB_ ( 12) -BP) activity assay. Results It was observed that IL-1 stimulates trans-cytokine B (CB)