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目的探讨模拟失重对背根神经节(dorsal root ganglia,DRG)及胶质细胞源性神经营养因子(glial cell line-derived neurotrophic factor,GDNF)的影响。方法健康雄性SD大鼠80只,随机分为尾部悬吊(HU)组(n=40)和正常对照(NC)组(n=40),4周后处死各组大鼠,取腰5背根神经节,甲苯氨兰染色观察背根神经节内尼氏小体变化,免疫组化观察GDNF的变化,Western-blot方法检测GDNF的蛋白表达,实时PCR检测GDNF m RNA表达情况。结果与NC组相比,HU组尼氏体染色浅,尼氏体变小,弥散分布。免疫组化结果显示,与NC组比较,HU组GDNF量及积分光密度(integral optical density,IOD)值减少(P<0.05)。Western-blot结果显示,HU组较NC组GDNF蛋白表达减少(P<0.05)。实时PCR结果显示,与NC组相比,HU组GDNF m RNA表达降低(P<0.05)。结论 4周模拟失重可导致DRG内尼氏小体形态发生变化,GDNF数量减少,GDNF蛋白表达及m RNA表达降低,推测失重状态下可引起大鼠背根神经节发生损伤。
Objective To investigate the effects of simulated weightlessness on dorsal root ganglia (DRG) and glial cell line-derived neurotrophic factor (GDNF). Methods Eighty healthy male Sprague-Dawley rats were randomly divided into 4 groups: tail suspension (n = 40) and normal control group (n = 40) Root ganglion and toluidine blue staining were used to observe the changes of Nissl body in the dorsal root ganglion. The changes of GDNF were observed by immunohistochemistry. The expression of GDNF was detected by Western-blot. The expression of GDNF m RNA was detected by real-time PCR. Results Compared with NC group, HU group Nissl staining was light, Nisshin smaller, diffuse distribution. The results of immunohistochemistry showed that the GDNF and integral optical density (IOD) of HU group were decreased (P <0.05) compared with NC group. Western-blot results showed that the expression of GDNF in HU group was lower than that in NC group (P <0.05). Real-time PCR results showed that the expression of GDNF m RNA in HU group was lower than that in NC group (P <0.05). CONCLUSIONS: Four weeks of simulated weightlessness can lead to the change of Nissl’s body morphology in DRG, the decrease of GDNF and the decrease of GDNF and m RNA expression. It is speculated that DRG can cause damage to DRG.