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介绍了10年生、15年生兴安落叶松不同单株及不同部位的当年生枝条上的休眠芽进行离体培养,诱导不定根形成的方法。筛选出生根率高的优良单株(生根率为10.0%)和生根培养基(SH+NAA0.5+IBA 0.5)。并证明了兴安落叶松树龄在10年及15年时植株间和每个单株的不同部位(上、中、下)间不定根形成无明显差异。
The dormant buds of perennial shoots of 10-year-old and 15-year-old Larix gmelinii were introduced in vitro to induce adventitious root formation. The excellent single rooted plants (rooting rate 10.0%) and rooting medium (SH + NAA 0.5 + IBA 0.5) were screened out. The results showed that there was no significant difference in the adventitious root formation between different plants (upper, middle and lower) between plants and each plant at 10 years and 15 years.