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目的:对南京地区庚型病毒性肝炎的临床和病原学特点进行分析。方法:用反转录聚合酶链反应(RT-PCR)法检测血清HGV-RNA。从274例病毒性肝炎患者中检测出17例庚型病毒性肝炎,观察其临床表现及血清病原学标志,并分析庚型肝炎病毒(HGV)部分核酸序列。结果与结论:17例患者男性成年人多见,全年散发,经输血感染为重要传播途径。HGV可以单独感染,也可重叠(混合)其它肝炎病毒感染。少数为急性肝炎,多数为慢性肝炎或肝硬化,尤其在重叠(混合)感染。单纯HGV感染者症状轻,多隐匿发病,肝功能损害较轻。重叠(混合)感染者多有慢性肝炎的症状,与HBV重叠(混合)感染时有形成重型肝炎的趋势。核酸序列分析表明HGV南京株部分核苷酸序列与HGV美国株HGU 44402、HGU 45966、HGU 36380及HGV河北株在对应位置的核苷酸同源性从87.27%~93.94%,可能HGV有不同的基因型。
Objective: To analyze the clinical and etiological characteristics of hepatitis G in Hepatitis of Nanjing. Methods: Serum HGV-RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Seventeen cases of hepatitis G virus were detected in 274 patients with viral hepatitis, and the clinical manifestations and serum etiological markers were observed. The partial nucleotide sequence of hepatitis G virus (HGV) was also analyzed. RESULTS AND CONCLUSION: Seventeen patients were more common in male adults and distributed throughout the year. Transfusion infection was an important route of transmission. HGV can be infected alone or in combination (mixed) with other hepatitis virus infections. A minority of acute hepatitis, the majority of chronic hepatitis or cirrhosis, especially in overlapping (mixed) infection. Simple HGV infection symptoms were mild, more occult disease, liver damage less. Overlapped (mixed) patients with chronic hepatitis symptoms often have overlap with HBV (mixed) infection with the tendency to develop severe hepatitis. Nucleotide sequence analysis showed that nucleotide homology between HGV Nanjing strain and HGV strain HGU 44402, HGU 45966, HGU 36380 and HGV Hebei strain was 87.27% ~ 93.94% genotype.