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用丙型肝炎病毒（ＨＣＶ）ＲＮＡ阳性的血清感染人类Ｔ淋巴细胞（Ｍｏｌｔ－４），１２小时后继续孵化１～２８天，将感染后的产物用聚合酶链反应（ＰＣＲ）测定，于第１、２、３天和第８天发现ＨＣＶ－ＲＮＡ的正链，第２和第８天发现ＨＣＶ－ＲＮＡ的负链。
Human T lymphocytes (Molt-4) were infected with hepatitis C virus (HCV) RNA-positive sera and continued to hatch for 1 to 28 days after 12 hours. The post-infection products were assayed by polymerase chain reaction (PCR) The positive strand of HCV-RNA was found on days 1, 2, 3, and 8 and negative on HCV-RNA on days 2 and 8.