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AIM:To investigate the expression of local renin andangiotensinogen mRNA in cirrhotic portal hypertensive patients.METHODS:The expression of local renin and angiotensinogenmRNA in the liver,splenic artery and vein of PH patientswas detected by RT-PCR analysis.RESULTS:Expression of local renin mRNA in the liver ofcontrol group was (0.19±0.11),significantly lower than thatin splenic artery(0.45±0.17)or splenic vein(0.39±0.12)respectively,(P<0.05).Expression of local angiotensinogenmRNA in the liver was (0.64±0.21),significantly higher thanthat in splenic artery(0.41±0.15) or in splenic vein (0.35±0.18)respectively,(P<0.05).Expression of local renin mRNA in theliver,splenic artery and vein of PH group was (0.78±0.28),(0.86±0.35) and (0.81±0.22) respectively,significantly higherthan that in the control group,(P<0.05).Expression of localangiotensinogen mRNA in the liver,splenic artery and vein ofPH group was (0.96±0.25),(0.83±0.18) and (0.79±0.23)respectively,significantly higher than that in the control group,(P<0.05).There was no significant difference between theliver,splenic artery and vein in the expression of local reninor local angiotensinogen mRNA in PH group,(P<0.05).CONCLUSION:In normal subjects the expression of localrenin and angiotensinogen mRNA was organ specific,butwith increase of the expression of LRAS,the organ-specificitybecame lost in cirrhotic patients.LRAS may contribute toincreased resistance of portal vein with liver and formationof splanchnic vasculopathy.
AIM: To investigate the expression of local renin and angiotensinogen mRNA in cirrhotic portal hypertensive patients. METHODS: The expression of local renin and angiotensinogen mRNA in the liver, splenic artery and vein of PH patients was detected by RT-PCR analysis .RESULTS: Expression of local renin mRNA in the liver of control group was (0.19 ± 0.11), significantly lower than that of splenic artery (0.45 ± 0.17) or splenic vein (0.39 ± 0.12) respectively, (P <0.05) .Expression of local angiotensinogen mRNA in the liver was ± 0.21), significantly higher thanthat in splenic artery (0.41 ± 0.15) or in splenic vein (0.35 ± 0.18) respectively, (P <0.05) .Expression of local renin mRNA in the liver, splenic artery and vein of PH group was Significant higherthan that in the control group, (P <0.05) .Expression of localangiotensinogen mRNA in the liver, splenic artery and vein of PH group was (0.96 ± 0.25) and (0.81 ± 0.22) ), (0.83 ± 0.18) and (0.79 ± 0.23) respectively, significantly highe r than that in the control group, (P <0.05). There was no significant difference between the liver, splenic artery and vein in the expression of local reninor local angiotensinogen mRNA in PH group, (P <0.05) .CONCLUSION: In normal subjects the expression of localrenin and angiotensinogen mRNA was organ specific, but with increase of the expression of LRAS, the organ-specificitybecame lost in cirrhotic patients. LRAS may contribute toincreased resistance of portal vein with liver and formationof splanchnic vasculopathy.