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利用日本沼虾13对中高多态性的微卫星位点分析了淮河安徽段日本沼虾野生群体的遗传结构。结果表明:11个群体中共有118个位点经Bonferroni校正后显示杂合不足且显著偏离Hardy-Weinberg平衡;群体平均期望杂合度均小于0.658,显示出较低的遗传多样性水平,其中瓦埠湖、焦岗湖群体遗传多样性相对较高,高塘湖群体相对较低;淮河安徽段日本沼虾群体偏离了突变-漂移平衡,呈现出遗传多样性下降的趋势,说明群体即将或曾经经历瓶颈效应;群体间AMOVA分析表明,群体间遗传分化程度较低(FST=0.011 6),且没有形成显著的遗传结构,在种质资源保护和管理上可视作一个单元,这为日本沼虾种质资源保护和合理开发利用提供了基础资料。研究亮点:日本沼虾是我国重要的淡水经济虾类,本研究分析了淮河安徽段日本沼虾野生群体的遗传结构,发现淮河安徽段日本沼虾野生群体遗传多样性及群体间遗传分化程度均较低,且没有形成明显的遗传结构,提出在种质资源保护和利用上可视为一个单元进行操作。
The genetic structure of the wild population of Macrobrachium nipponensis in Anhui section of Huaihe River was analyzed using 13 high and low polymorphism microsatellite loci in Macrobrachium nipponense. The results showed that a total of 118 loci in 11 populations showed heterozygosity after being corrected by Bonferroni, which deviated significantly from Hardy-Weinberg equilibrium. The average expected heterozygosity of the population was less than 0.658, indicating a lower level of genetic diversity, The genetic diversity of lake and Jiaoogang Lake was relatively high, and the population of Gaotang Lake was relatively low. The population of M. matsudana in Anhui section deviated from the balance of mutation-drift, showing the trend of decline in genetic diversity, indicating that the population is about to or have experienced The results of AMOVA showed that there was a low degree of genetic differentiation among populations (FST = 0.011 6), and no significant genetic structure was formed. It was considered as a unit in the conservation and management of germplasm resources, Germplasm resources protection and rational development and utilization provide the basic information. Research highlights: Macrobrachium nipponense is an important freshwater economic shrimp in China. In this study, the genetic structure of the wild population of Macrobrachium nipponense in Anhui section of the Huaihe River was analyzed. The genetic diversity of the wild population of Macrobrachium nipponense in Anhui section of Anhui Province and the genetic differentiation among the populations were found Low, and did not form a clear genetic structure, proposed conservation and utilization of germplasm resources can be regarded as a unit to operate.