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目的:探讨桂附地黄丸抗大鼠肺纤维化的作用及对外周血CD4、CD8T淋巴细胞亚群及CD4/CD8的影响。方法:48只Wister大鼠随机分为正常对照组(12只),模型对照组(12只),泼尼松对照组(12只)、桂附地黄丸组(12只)。采用气管内一次性注入平阳霉素复制大鼠肺纤维化病理模型。14 d开始用药组每天灌胃桂附地黄丸,28 d后处死各组大鼠。HE染色显微镜下观察各组大鼠肺组织病理变化;VG染色观察各组大鼠肺纤维化程度;免疫组化法检测各组大鼠肺组织中肿瘤坏死因子(TNF-α)的表达;流式细胞术检测外周血淋巴细胞CD4、CD8和CD4/CD8的表达。结果:模型组大鼠肺纤维化程度、肺组织中TNF-α表达均明显高于正常组、桂附地黄丸组及泼尼松对照组(P<0.05);桂附地黄丸组CD4和CD4/CD8比值显著高于模型对照组(P<0.05)。结论:桂附地黄丸能明显减轻大鼠肺纤维化程度,其机制可能与抑制TNF-α表达有关;同时桂附地黄丸还能提高外周血CD4和CD4/CD8比值。
Objective: To investigate the effect of Gui Fu Di Huang Wan on pulmonary fibrosis in rats and its effect on CD4, CD8 T lymphocyte subsets and CD4 / CD8 in peripheral blood. Methods: Forty-eight Wister rats were randomly divided into normal control group (n = 12), model control group (n = 12), prednisone control group (n = 12) and Guifudihuangwan group (n = 12). Pulmonary fibrosis was induced by intratracheal instillation of bleomycin. On the 14th day, the rats in the medication group were given Guifu Dihuang Pills every day, and the rats in each group were sacrificed 28 days later. The pathological changes of lung tissue were observed under HE staining microscope. The degree of pulmonary fibrosis was observed by VG staining. The expression of tumor necrosis factor (TNF-α) in lung tissue of each group was detected by immunohistochemistry. Cytometry was used to detect the expression of CD4, CD8 and CD4 / CD8 in peripheral blood lymphocytes. Results: The degree of pulmonary fibrosis and the expression of TNF-α in lung tissue in model group were significantly higher than those in normal group, Guifu Dihuang Pills group and prednisone control group (P <0.05) / CD8 ratio was significantly higher than the model control group (P <0.05). Conclusion: Gui Fu Di Huang Wan can significantly reduce the degree of pulmonary fibrosis in rats, the mechanism may be related to the inhibition of TNF-α expression; at the same time Gui Fu Di Huang Wan also increase the ratio of CD4 and CD4 / CD8 in peripheral blood.