论文部分内容阅读
目的:采用LC-HR-MS/MS技术研究紫草中酚酸类化学成分。方法:采用LC-HR-MS/MS法,使用C18(4.6 mm×100 mm,1.8μm)色谱柱,以乙腈-0.1%甲酸溶液为流动相,梯度洗脱,流速0.4 m L·min~(-1),柱温30℃,进样量10μL;质谱条件:HESI离子源;正负离子扫描模式;雾化温度为300℃;离子传输管温度为380℃,质量扫描范围为m/z 100~1 500。结果:以酚酸类化合物的质谱裂解规律为指导,参照紫草中已报道成分的质谱数据,分析推断了紫草中可能含有紫草酸、咖啡酸四聚体、迷迭香酸、和丹酚酸C等9个化学成分。结论:本实验建立的分析方法能够较全面地反映出紫草中酚酸类的化学成分,为进一步开发利用该药材提供实验依据。
Objective: To study the phenolic acids in Lithospermum officinale by LC-HR-MS / MS. Methods: The mobile phase consisted of acetonitrile-0.1% formic acid and gradient elution at a flow rate of 0.4 m L · min ~ (-1) using LC-HR-MS / MS with C18 (4.6 mm × 100 mm, 1.8 μm) -1), column temperature 30 ℃, injection volume 10μL; mass spectrometry conditions: HESI ion source; positive and negative ion scanning mode; atomization temperature of 300 ℃; ion transfer tube temperature of 380 ℃, mass scan range of m / z 100 ~ 1 500. Results: Based on the mass spectrometric cleavage of phenolic acids and the mass spectral data of the reported components in Lithospermum indicum, it was concluded that Lithospermum officinale may contain shikonin, caffeic acid tetramer, rosmarinic acid, Acid C 9 chemical composition. Conclusion: The analytical method established in this experiment can reflect the chemical constituents of phenolic acids in Lithospermum more comprehensively and provide the experimental basis for further development and utilization of the medicinal materials.