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目的为了制备能检测日本血吸虫种内多态性的ｒＤＮＡ探针，以用于不同地域日本血吸虫品系间差异的研究。方法根据曼氏血吸虫大、小亚基的基因序列，设计引物，ＰＣＲ扩增得到４．４ｋｂ的日本血吸虫中国大陆株安徽品系的ｒＤＮＡ片段，经纯化后克隆入ｐＵＣ１８质粒。结果得到了２个转化成功的含日本血吸虫中国大陆株ｒＤＮＡ基因的重组质粒的ＪＭ１０３菌落。结论我们采用的方法，使获取目的基因片段的手段更为简单、方便。
Objective To prepare rDNA probes capable of detecting intraspecific polymorphism of Schistosoma japonicum for studying the differences between strains of Schistosoma japonicum in different regions. Methods According to the gene sequences of large and small subunits of Schistosoma mansoni, primers were designed and the rDNA fragment of 4.4kb of Anhui strain of Schistosoma japonicum was amplified by PCR. After cloning, the rDNA fragment was cloned into pUC18 plasmid. RESULTS: Two JM103 colonies of recombinant plasmid containing Chinese rDNA gene of Schistosoma japonicum were successfully transformed. Conclusions We used a method that made it easier and more convenient to obtain the target gene fragment.