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目的 评价芥子气全身吸收中毒后对生殖器官的中、长期影响。方法 以成年家犬为中毒模型 ,雌雄各半 ,中毒剂量为 12mg/kg ,皮下注射。治疗组动物在中毒后 30min即进行相应的药物治疗。对死亡动物及经治疗后存活动物 (中毒后 11d及 2 8d各活杀 1条 )进行剖验 ,重点观察生殖器官 (睾丸和卵巢 )在光镜下的组织学改变。结果 中毒组犬于 3d内死亡 3条 ,治疗组犬全部成活。病理切片显示雌性犬卵巢损伤在治疗后 1个月内能基本恢复正常 ,而芥子气对雄性犬睾丸的损伤具有较强的远期效应。中毒治疗后 2 8d犬的各级精原细胞明显减少 ,观察不到成熟精子 ,而中毒后 3d内死亡犬的生精细胞损伤相对较轻。结论 芥子气对雄性生殖细胞具有明显的远期毒性效应 ,一般治疗措施并不能在短时间内有效的促进生殖细胞的恢复
Objective To evaluate the medium and long term effects of systemic absorption of mustard on reproductive organs. Methods Adult domestic dogs as poisoning model, male and female, poisoning dose of 12mg / kg, subcutaneous injection. The animals in the treatment group were treated with the corresponding drugs 30 minutes after the poisoning. The necrotic animals and the survival of animals after treatment (poisoned 11d and 28d kill one) to conduct anatomy, highlighting the reproductive organs (testes and ovaries) histological changes under light microscopy. Results The poisoning group died 3 dogs in 3d, all dogs in treatment group survived. Pathological examination showed that ovine injury in female dogs returned to normal within 1 month after treatment, and mustard had a strong long-term effect on the damage of testis in male dogs. After 28 days of poisoning, spermatogonia of dogs at all levels were significantly reduced, and mature spermatozoa were not observed. However, spermatogenic cells of the deceased dogs were relatively less damaged after 3 days of poisoning. Conclusion Mustard gas has obvious long-term toxic effect on male germ cells, and the general treatment measures can not effectively promote the recovery of germ cells in a short time