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以人心肌肌钙蛋白Ｔ（ｃＴｎＴ）为抗原，采用脾内免疫法，免疫ＢＡＬＢ／ｃ小鼠，取其脾淋巴细胞与小鼠Ｓｐ２／０细胞融合，经间接ＥＬＩＳＡ法筛选，三次克隆化后获得５株能稳定分泌抗ｃＴｎＴ单克隆抗体（ＭｃＡｂ）的杂交瘤细胞Ｇ３、Ｇ８、Ｇ１０、Ａ５和Ａ７。免疫球蛋白亚类鉴定其中１株为ＩｇＧ２ａ，４株为ＩｇＭ。染色体数目９２～１１０条。将Ｇ３、Ｇ８、Ｇ１０、Ａ５的单克隆抗体腹水做１：１００稀释与ＬＤＨ、ＣＫ、ＣＫＭＢ和ＧＯＴ等心肌酶均无交叉反应。５株ＭｃＡｂ的腹水效价为３．２×１０－６～１．６×１０－７。ＭｃＡｂ相加试验表明，Ａ５和Ｇ３可识别不同的抗原表位。
BALB / c mice were immunized with cTnT as antigen and spleen lymphocytes were fused with mouse Sp2 / 0 cells by indirect immunosorbent assay. After three clones Five hybridoma cells G3, G8, G10, A5 and A7 that can stably secrete anti-cTnT monoclonal antibody (McAb) were obtained. Immunoglobulin subclass identification of which one is IgG2a, 4 strains of IgM. Chromosome number 92 ~ 110. The G3, G8, G10, A5 monoclonal antibody ascites do 1: 100 dilution and LDH, CK, CKMB and GOT and other myocardial enzymes did not cross-react. The ascites titer of 5 McAbs was 3.2 × 10-6 ~ 1.6 × 10-7. McAb addition experiments show that A5 and G3 can recognize different epitopes.