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目的:观察胃癌患者血管内皮生长因子C和D(vascular endothelial growth factor-Cand-D,VEGF-C,VEGF-D)在胃癌组织中的表达以及其与肿瘤微血管生成的关系。方法:采用免疫组化S-P法检测30例胃癌组织和30例正常胃组织中VEGF-C和VEGF-D蛋白的表达,检测微血管密度(microvessel density,MVD)。结果:胃癌组织VEGF-C和VEGF-D表达明显高于正常胃组织(p<0.01),其中淋巴结转移组VEGF-C、VEGF-D的表达与淋巴结未转移组间差异显著(p<0.05)。胃癌组织MVD值明显高于正常胃组织(p<0.01),淋巴结转移组MVD值明显高于未转移组(p<0.01)。MVD与VEGF-C的蛋白在胃癌组织中的表达高度相关(r=0.735,p<0.05),与VEGF-D的蛋白在胃癌组织中的表达成正相关(r=0.623,p<0.05)。结论:VEGF-C和VEGF-D的高表达与肿瘤微血管生成及淋巴道转移密切相关,可作为评估胃癌患者预后的重要参考指标。
OBJECTIVE: To observe the expression of vascular endothelial growth factor-C and vascular endothelial growth factor-C (VEGF-C) and vascular endothelial growth factor-C (VEGF-D) in gastric cancer and its relationship with tumor angiogenesis. Methods: The expressions of VEGF-C and VEGF-D in 30 cases of gastric carcinoma and 30 cases of normal gastric tissues were detected by immunohistochemical S-P method, and the microvessel density (MVD) was detected. Results: The expression of VEGF-C and VEGF-D in gastric cancer tissues was significantly higher than that in normal gastric tissues (p <0.01). There was significant difference between the expression of VEGF-C and VEGF-D in lymph node metastasis and non- . MVD in gastric cancer tissues was significantly higher than that in normal gastric tissues (p <0.01). The MVD in lymph node metastasis group was significantly higher than that in non-metastasis group (p <0.01). MVD and VEGF-C protein expression in gastric cancer was highly correlated (r = 0.735, p <0.05), and VEGF-D protein expression in gastric cancer was positively correlated (r = 0.623, p <0.05). Conclusion: The overexpression of VEGF-C and VEGF-D is closely related to tumor angiogenesis and lymphatic metastasis, which may be used as an important reference to evaluate the prognosis of patients with gastric cancer.