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为研究三磷酸腺苷二钠对慢性高原病(chronic mountain sickness,CMS)大鼠肝脏的保护作用,取60只SD大鼠,对其中50例进行慢性高原病造模后,随机分为高原模型组、硝苯地平对照组、三磷酸腺苷二钠低剂量组、三磷酸腺苷二钠中剂量组、三磷酸腺苷二钠高剂量组,每组10只;剩余10只平原环境饲养为正常对照组;造模30 d后,根据大鼠体重进行干预。连续灌胃15d后,测定肺动脉压(pulmonary artery pressure,PAP)、同型半胱氨酸(homocysteine,Hcy)、白介素-6(interleukin-6,IL-6)、C-反应蛋白(C-reactionprotein,CRP)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)、谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate transaminase,AST)等指标。结果发现,与正常对照组比较,高原模型组和硝苯地平对照组大鼠体内的Hcy,IL-6,CRP,MDA,ALT和AST水平升高,SOD和GSH-PX水平降低,PAP升高,提示高原低氧对大鼠的肝脏造成损害。与高原模型组比较,三磷酸腺苷二钠高剂量组大鼠体内的Hcy,IL-6,CRP,MDA,ALT和AST水平降低(P<0.05),SOD和GSH-PX水平升高(P<0.05)。故而认为高剂量的三磷酸腺苷二钠对CMS大鼠肝脏有保护作用。
In order to study the protective effect of adenosine triphosphate (ATP) on the liver of rats with chronic mountain sickness (CMS), 60 SD rats were selected and 50 of them were divided into plateau model group, Benzene terbinafine group, low dose of adenosine triphosphate, middle dose of adenosine triphosphate and high dose of adenosine triphosphate, with 10 in each group. The remaining 10 plains were kept as normal control group. After 30 days of modeling, Rat body weight intervention. Pulmonary artery pressure (PAP), homocysteine (Hcy), interleukin-6 (IL-6) and C-reaction protein CRP, superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-PX), alanine aminotransferase (ALT) , Aspartate transaminase (AST) and other indicators. The results showed that the levels of Hcy, IL-6, CRP, MDA, ALT and AST, the levels of SOD and GSH-PX in the model group and the nifedipine control group were significantly lower than those in the normal control group , Suggesting that high altitude hypoxia caused damage to the liver of rats. The levels of Hcy, IL-6, CRP, MDA, ALT and AST were significantly decreased (P <0.05) and the levels of SOD and GSH-PX were significantly increased in high dose sodium adenosine triphosphate group compared with the model group . Therefore, high dose of adenosine triphosphate (BDNF) is considered to have a protective effect on the liver of CMS rats.