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该文采用超声法制备一种新型可降解、生物相容性良好的负载姜黄素(curumin,Cur)红细胞膜纳米粒子(Cur-RBCNPs)。通过比较不同参数制备所得纳米粒子的粒径来优化Cur-RBC-NPs的制备条件,并考察其形态、粒径分布、稳定性、体外释放特性、细胞对纳米粒子的摄取及体外抗肿瘤效果等。结果表明,所制得姜黄素纳米粒子为较规则的圆球形,平均粒径为(245.7±1.3)nm,包封率为50.65%±1.36%,载药量为6.27%±0.29%,具有良好的缓释特性。体外实验中,纳米粒子能够被肿瘤细胞迅速摄取,Cur-RBC-NPs组有显著的抑制肿瘤生长效果。负载姜黄素的红细胞膜纳米粒子制备方法简便,具有良好的药物缓释性和抗肿瘤能力,有望成为新型的有临床应用前景的纳米给药系统。
In this paper, a novel biodegradable, curcumin (Cur-loaded) erythrocyte membrane nanoparticles (Cur-RBCNPs) with good biocompatibility were prepared by ultrasonic method. The preparation conditions of Cur-RBC-NPs were optimized by comparing the particle sizes of the prepared nanoparticles with different parameters, and their morphology, particle size distribution, stability, in vitro release characteristics, cell uptake of nanoparticles and antitumor effects in vitro . The results showed that the prepared curcumin nanoparticles were relatively spherical with the average diameter of (245.7 ± 1.3) nm, encapsulation efficiency of 50.65% ± 1.36% and drug loading of 6.27% ± 0.29% Release characteristics. In vitro experiments, the nanoparticles can be quickly taken up by tumor cells, Cur-RBC-NPs group significantly inhibited tumor growth. The preparation of curcumin-loaded erythrocyte membrane nanoparticles is simple, has good drug release and anti-tumor ability, and is expected to become a new nano-drug delivery system with clinical application prospect.