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以美洲大蠊 (Periplanata americana)雄性成虫的中枢神经系统为试材 ,对 Na+ -K+ - ATPase作为农药筛选靶标进行了初步研究 ,表明 Na+ - K+ - ATPase活力测定的最适反应条件是 :酶源蛋白浓度 8.5μg/ m L,温度 30℃ ,p H7.4 ,反应时间 15min。同时以最佳反应系统初步研究了几种拟除虫菊酯 (南开菊酯、氯氰菊酯、溴灭菊酯、氰戊菊酯、二氯苯醚菊酯、胺菊酯 )对 Na+ - K+ - ATPase离体活性的影响 ,结果显示出不同程度的抑制作用。并对其用作农药筛选靶标的可能性进行了探讨。
Taking the central nervous system of adult male Periplaneta americana as test material, Na + -K + - ATPase was selected as the target of pesticide screening. The optimum reaction conditions of Na + - K + - ATPase activity determination were as follows: enzyme source Protein concentration 8.5μg / m L, temperature 30 ℃, p H7.4, reaction time 15min. In the meantime, the effects of several pyrethroids (SMF, cypermethrin, fenvalerate, fenvalerate, permethrin, tetramethrin) on the activity of Na + - K + - ATPase in vitro Activity, the results showed different degrees of inhibition. The possibility of using it as a pesticide screening target was also discussed.