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目的:观察碱性成纤维细胞生长因子（bFGF）处理对脂肪间充质干细胞(ADSCs)治疗肝硬化大鼠疗效的影响。方法:采用四氯化碳腹腔注射10周诱导建立大鼠肝硬化模型。30只SD大鼠随机分为3组（n n = 10），对照组：尾静脉注射磷酸盐缓冲液（PBS） 0.5 ml；单纯ADSCs移植组（ADSCs组）：尾静脉移植1×10n 6个ADSCs；bFGF处理的ADSCs治疗组[ADSCs（bFGF）组]：尾静脉移植1×10n 6个bFGF处理的ADSCs。移植后1周检测肝功能、病理改变和细胞因子变化并监测移植细胞体内存活转化情况。统计学方法采用独立样本n t检验和方差分析。n 结果:bFGF处理可明显促进ADSCs的增殖、分化以及过表达肝细胞生长因子（HGF）[ADSCs：（2137.16±261.52）pg/ml比ADSCs（bFGF）：（4 776.23±532.44）pg/ml，n P < 0.05)，bFGF处理的ADSCs治疗组在促进肝功能恢复方面差异有统计学意义[丙氨酸转移酶（ALT）：ADSCs （190.8±34.98）U/L比ADSCs（bFGF）：（117.8±35.81）U/L;天冬氨酸转移酶（AST）：ADSCs （295.2±33.71）U/L比ADSCs（bFGF）：（183.8±41.29）U/L， n P值均< 0.05]，PBS组和单纯ADSCs组以及单纯ADSCs组和ADSCs（bFGF）组组间血清白蛋白水平差异无统计学意义，而相比于PBS组，ADSCs（bFGF）组血清白蛋白水平明显升高（n P < 0.05），改善肝再生和减轻肝损伤方面较PBS对照组和单纯ADSCs移植组差异有统计学意义（ n P < 0.05）；Masson trichrome染色显示bFGF处理的ADSCs治疗组肝纤维化面积百分比为6.78%±0.56%，明显高于PBS对照组和单纯ADSCs移植组（7.96%±0.64%）（ n P < 0.05)；蛋白质印迹法分析显示bFGF处理的ADSCs治疗组肝组织HGF蛋白表达明显上调，而α-平滑肌肌动蛋白表达明显下调，与单纯ADSCs移植组存在明显差异，双重荧光染色法显示bFGF处理的ADSCs治疗组肝组织中干细胞植入数要高于单纯ADSCs移植组。体外细胞实验证实bFGF可明显促进ADSCs过表达HGF。n 结论:bFGF处理的ADSCs移植可明显改善肝硬化大鼠的肝功能和纤维化。疗效优于ADSCs单独移植治疗。“,”Objective:To observe the effect of basic fibroblast growth factor (bFGF) treatment on efficacy of adipose-derived mesenchymal stem cells (ADSCs) in cirrhotic rats.Methods:A rat model of liver cirrhosis was established via intraperitoneal injection of carbon tetrachloride (CCln 4) for 10 weeks. Thirty SD rats were randomly divided into 3 groups (n n = 10): control group served as group A, and 0.5ml of phosphate-buffered saline (PBS) was transfused into the tail vein; ADSCs single-dose transplantation group served as group B, and 1×10n 6 ADSCs were transplanted into the tail vein; bFGF-treated ADSCs treatment group served as group C, and 1×10n 6 bFGF-treated ADSCs were transplanted through tail vein. Liver function, pathological and cytokine changes, and the in vivo survival transformation condition of the transplanted cells were measured at one week after transplantation. F test and an independent sample t test were used.n Results:bFGF treatment had significantly promoted the proliferation, differentiation and overexpression of hepatocyte growth factor (HGF) in ADSCs [ADSCs single: (2 137.16 ± 261.52) pg/ml vs. ADSCs (bFGF): (4 776.23 ± 532.44) pg/ml, n P < 0.05]. The bFGF-treated ADSCs treatment group had statistically significant differences in promoting the recovery of liver function [alanine aminotransferase (ALT): ADSCs single (190.8 ± 34.98) vs. ADSCs (bFGF): (117.8 ± 35.81) pg/ml; aspartate aminotransferase (AST): ADSCs single (295.2 ± 33.71) U/L vs. ADSCs (bFGF): (183.8 ± 41.29) U/L, n P ??< 0.05). There was no statistically significant difference in serum albumin levels between the control group, ADSCs single group, and ADSCs (bFGF) group. Compared with the control group, the serum albumin level of ADSCs (bFGF) group was increased significantly (n P < 0.05), and the difference between the control group and the ADSCs single transplantation group in improving liver regeneration and reducing liver damage was statistically significant ( n P < 0.05). Masson trichrome staining showed that the percentage of the liver fibrosis area in the bFGF-treated ADSCs treatment group was 6.78% ± 0.56%, which was significantly higher than that of the control and ADSCs single dose transplantation group (7.96% ± 0.64%) ( n P < 0.05). Western blot analysis showed that the expression of HGF protein in the bFGF-treated ADSCs treatment group was significantly up-regulated, while the expression of α-smooth muscle actin was significantly down-regulated, and the difference was significant in the ADSCs single transplantation group. A double fluorescent staining method showed that the numbers of stem cells implanted in the liver tissue of the bFGF-treated ADSCs treatment group were higher than that of the ADSCs single transplantation group. In-vitro cell experiments confirmed that bFGF had significantly promoted the overexpression of HGF in ADSCs.n Conclusion:bFGF-treated ADSCs transplantation can significantly improve liver function and fibrosis as compared with ADSCs single-dose transplantation in cirrhotic rats.