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本实验采用垂直平板聚丙烯酰胺凝胶电泳法,比较分析了我国12个省区的25个杉木种源的过氧化物酶同工酶,所得结果表明: 1、杉木种源在南北种植区表达的同工酶谱具有普遍性,其总趋势是:南部种植区酶谱类型简单,带少、活性弱,多数酶带着色浅而带型较宽;北部种植区酶谱类型丰富,酶带多且活性强。 2、四川种植区内的杉木种源酶谱表达,也符合南北种植区的酶谱变化规律,但总是较南部种植区表达了较多的酶带,且多数酶带的活性较强。 3、高纬度、低纬度,以及高海拔、低海拔的种源,它们在各个种植区内的酶谱表达,也服从于南北表达规律,这就充分显示了同工酶——环境交互作用的生化基础。
In this experiment, vertical plate polyacrylamide gel electrophoresis was used to analyze the peroxidase isozymes of 25 Chinese fir provenance in 12 provinces and autonomous regions in China. The results showed that: 1, Cunninghamia lanceolata The general trend is: isozyme type is simple in southern planting area, with less, activity is weak, most of the enzyme with light colored and wide band; the northern part of the planting area is rich in zymogram, with more enzyme And activity. The zymogram expression of Chinese fir seed in Sichuan planting area also accorded with the law of zymogram changes in the south and north growing areas, but it was always more than that in the southern planting area, and the activity of most zymograms was stronger. 3, high latitude, low latitude, and high altitude, low altitude provenance, their zymogram expression in various planting areas, but also subject to the North-South law of expression, which fully demonstrated the isoenzyme-environment interaction Biochemical basis.