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常规制取注射性胶原的方法是用酶降解法，经反复酸提、盐析、离心、交联等步骤，进行纯化，该法将胶原降解，酸提取分子片断，用交联剂组成可注射胶原。本法是从ＮＥＣＭ开始制取，ＮＥＣＭ已为胶原框架，因此步骤简化很多，仅需解裂胶原网状结构，酶解末端蛋白以消除特异性，最后降解成３００ｎｍ左右胶原。本法不用交联剂和稳定剂。生物原材料来源广泛，有利临床推广应用。经半年动物实验证明，本法制取的可注射胶原无排斥反应和炎性反应，也无包裹现象，半年时仍残留有部分胶原
Conventional Preparation of injectable collagen method is enzymatic degradation method, after repeated acid extraction, salting out, centrifugation, crosslinking and other steps, the purification, the method of collagen degradation, acid extraction of molecular fragments, with a cross-linking agent composition can be injected collagen. This method is from the beginning of NECM, NECM has a collagen framework, so the steps are much simplified, just to crack the collagen network structure, end protein to eliminate specificity, and finally degraded into about 300nm collagen. This law does not require crosslinkers and stabilizers. Wide range of biological raw materials, favorable clinical application. After six months of animal experiments proved that the law can be made of injectable collagen non-rejection reaction and inflammatory response, no parcel phenomenon, six months still remained part of the collagen