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在高效液和色谱仪中,保持柱温的恒定相当重要。柱温对移动相的粘度和溶解性能,色谱传质过程以及固定相(包括载体)的物化性能等都有一定的影响。柱温波动会使组份保留时间不易重复,基线发生漂移,甚至使仪器不能在高灵敏度下做微量检测。适当提高柱温能降低移动相的粘度和柱前压,在梯度淋洗及离子对色谱过程中还能缩短物理和化学平衡时间,从而能提高仪器的效能。但有些仪器没有柱
In HPLC and chromatographs, it is important to keep the column temperature constant. The column temperature has a certain influence on the viscosity and solubility of the mobile phase, the chromatographic mass transfer process, and the physicochemical properties of the stationary phase (including the carrier). Fluctuations in the column temperature will make the retention time of the components difficult to repeat, the baseline drift occurs, and even make the instrument can not trace detection under high sensitivity. Appropriately increasing the column temperature can reduce the viscosity and column pressure of the mobile phase, and can shorten the physical and chemical equilibrium time during gradient elution and ion-pair chromatography, which can improve the performance of the instrument. But some instruments do not have columns