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目的探讨食管癌组织及癌旁组织中叶酸受体α的表达情况及其与食管癌临床病理特征的关系。方法取40例食管癌患者的癌组织及癌旁组织,应用免疫组化方法及即时定量逆转录PCR(real-time RT-PCR)检测其叶酸受体α(FRα)的表达。结果免疫组化染色结果显示,癌组织与癌旁组织比较FRα表达较低(P<0.01)。RT-PCR结果显示,80.0%(32/40)食管癌组织中FRα表达降低,17.5%(7/40)食管癌组织中FRα表达无变化,2.5%(1/40)表达增高;年龄<60岁比年龄≥60岁的食管癌组织FRα表达水平降低更为明显,两者比较差异有显著性(P=0.001)。FRα的表达与患者的性别以及食管癌临床病理学特征无显著相关性(P>0.05)。结论食管癌癌组织与癌旁组织比较FRα表达较低,癌组织FRα表达的降低随年龄的增长而更加明显,但与癌症的病变部位、病理类型、TNM分期、肿瘤大小、浸润深度、有无淋巴结转移无关。
Objective To investigate the expression of folic acid receptor α in esophageal cancer tissue and adjacent tissues and its relationship with the clinicopathological features of esophageal cancer. Methods Forty esophageal cancer tissues and adjacent tissues were obtained. The expression of folic acid receptor α (FRα) was detected by immunohistochemistry and real-time reverse transcription-polymerase chain reaction (RT-PCR). Results The results of immunohistochemistry showed that the expression of FRα was lower (P <0.01) than the adjacent tissues. The results of RT-PCR showed that the expression of FRα decreased in 80.0% (32/40) of esophageal cancer tissues, while the expression of FRα in 17.5% (7/40) of esophageal cancer tissues had no change, while the expression of FRa in 2.5% (1/40) The expression of FRα in esophageal cancer tissues was significantly lower than that in patients older than 60 years old (P = 0.001). There was no significant correlation between the expression of FRα and the gender of patients and the clinicopathological characteristics of esophageal cancer (P> 0.05). Conclusion The expression of FRα in esophageal cancer tissues is lower than that in adjacent non-cancerous tissues. The decrease of FRα expression in cancerous tissues is more obvious with the increase of age. However, the expression of FRα in esophageal cancer tissues is not correlated with the pathological type, TNM stage, tumor size, depth of invasion, Lymph node metastasis has nothing to do.