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Objective:To investigate the gene related to β-lactam resistance and to confirm the mechanism about a synergy effect between CPZ and β-lactam antibiotics.Methods:To measure antibacterial activity,we performed a minimum inhibitory concentration(MIC) and synergy test.Transmission electron microscopy(TEM) was used in morphological analysis.To analyze gene expression,we conducted reverse transcriptase polymerase chain reaction(PCR).Results:We confirmed a synergy effect between CPZ and β-lactam antibiotics.Furthermore,we observed that CPZ affect the cell envelope of MRS A by using TEM.At the gene level,CPZ reduced the expression of resistance genes.Conclusions:Through this result,we hypothesize that a decrease of resistance factor expressions was caused by CPZ because it disrupts the activity of a sensor protein located in the cell membrane.
Objective: To investigate the gene related to β-lactam resistance and to confirm the mechanism about a synergy effect between CPZ and β-lactam antibiotics. Methods: To measure antibacterial activity, we performed a minimum inhibitory concentration (MIC) and synergy test. electron microscopy (TEM) was used in morphological analysis. analyze gene expression, we conducted reverse transcriptase polymerase chain reaction (PCR). Results: We confirmed a synergy effect between CPZ and β-lactam antibiotics. cell envelope of MRS A by using TEM. At the gene level, CPZ reduced the expression of resistance genes.Conclusions: Through this result, we hypothesize that a decrease of resistance factor expressions was caused by CPZ because it disrupts the activity of a sensor protein located in the cell membrane.