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A novel determination method of Ag+ was established. In acetic acid-sodium acetate buffer (pH 5.0) medium, Ag+ reacts with SCN- to form AgSCN in the presence of TritonX-100,which results in an increase of resonance light scattering (RLS) and giving a new RLS spectrum.The maximum RLS peak was at 585 nm. The enhancement of resonance light scattering at 585nm was proportional to the concentration of Ag+ ranging from 0.0045-4.00 μg mL-1 (r=0.9991),and the detection limit was 1.37 ng mL-1 with the recovery of 97.70%～104.80%.