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目的 探讨有丝分裂激活的蛋白激酶 (MAPK)的三个亚族p38,ERK1及JNK1在非小细胞肺癌中表达的意义及ras与三者之间的关系,并判断它们对非小细胞肺癌预后的影响。方法 对 1992至 1997年共 73个患者的手术切除的标本蜡块行p38,ERK1,JNK1及ras的免疫组织化学分析,并判断p38,ERK1,JNK1与一些临床病理因素的关系,明确MAPK的不同亚族与ras的相关性,了解影响非小细胞肺癌预后的指标。结果 p38表达与淋巴结转移 (P=0 .000)和TNM分期有关 (P=0 .001)。JNK1表达与病理类型(P=0. 015 )、淋巴结转移 (P=0 .000 )、分期有关 (P=0 .000 ),ERK1表达与肿瘤位置有关(P=0 .005)。p38(P=0 .003)、ERK1(P=0. 012)显示与ras有相关性。单因素分析显示TNM分期 (P= 0. 0000 )、淋巴结转移 (P= 0 .0000 )、肿瘤分化 (P= 0 .0000 )、p38 (P=0. 0001)、JNK1(P=0 0232)及ras(P=0 0022)与非小细胞肺癌预后有关。多因素分析显示p38阴性(P=0 035)、临床分期为Ⅰ期 (P=0. 026 )、淋巴结转移阴性 (P=0 .044 )和肿瘤分化较好 (P=0 .020)的患者预后较好。结论 在MAPK的亚族中,p38的表达可以用来评价淋巴结转移和TNM分期,ERK1表达可评估病理类型、淋巴结转移和分期,JNK1与肿瘤位置有关。ras可能促进p38和ERK1的表达,p38的表达和肿瘤分化、?
Objective To investigate the significance of the expression of p38, ERK1 and JNK1, three subfamilies of mitogen-activated protein kinase (MAPK) in non-small cell lung cancer (NSCLC) and the relationship between ras and the three subtypes and to determine their effect on prognosis of non-small cell lung cancer . Methods Immunohistochemical analysis of p38, ERK1, JNK1 and ras in paraffin-embedded specimens of 73 patients from 1992 to 1997 was performed. The relationship between p38, ERK1, JNK1 and some clinicopathological factors was also analyzed. Sub-ras and ras related to understand the prognosis of non-small cell lung cancer indicators. Results The expression of p38 was correlated with lymph node metastasis (P = 0.000) and TNM staging (P = 0.001). The expression of JNK1 was correlated with the pathological type (P = 0.015), lymph node metastasis (P = 0.000) and staging (P = 0.000). The expression of ERK1 was correlated with tumor location (P = .005). p38 (P = .003), ERK1 (P = 0.012) showed a correlation with ras. Univariate analysis showed that TNM stage (P = 0.0000), lymph node metastasis (P = 0.0000), tumor differentiation (P = 0.0000), p38 (P = 0.0001), JNK1 And ras (P = 0 0022) were associated with the prognosis of non-small cell lung cancer. Multivariate analysis showed that patients with p38 negative (P = 0 035), clinical stage Ⅰ (P = 0.026), negative lymph node metastasis (P = 0.044) and well-differentiated tumors (P = 0.020) The prognosis is good. Conclusion In the subfamily of MAPK, the expression of p38 can be used to evaluate the lymph node metastasis and TNM stage. The expression of ERK1 can evaluate the pathological type, lymph node metastasis and staging, and JNK1 is related to tumor location. ras may promote the expression of p38 and ERK1, p38 expression and tumor differentiation,?