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目的 探讨激肽释放酶9(KLK9)基因在肝癌发生发展中的作用及其临床意义.方法 通过免疫组化和实时PCR法检测KLK9基因在肝癌细胞株和肝癌组织中的表达.结果 KLK9蛋白质在肝细胞癌组织中的表达率较癌旁组织和正常肝脏组织中明显升高(P＜0.05);KLK9mRNA在肝癌细胞株中的表达水平较正常肝细胞株明显升高(P ＜0.05);KLK9表达与肝癌是否转移、肿瘤的大小、分化程度、临床分期相关(P≤0.05),与患者的年龄、乙肝表面抗原、性别情况无关(P＞0.05).结论 KLK9基因在肝癌的发生发展中起重要作用,可作为肝癌标志物和预后判断的重要指标.本研究也有可能为肝癌的诊断及基因靶向治疗提供了新的靶点及理论基础.“,”Objective To investigate the expression of kallikrein 9 (KLK9) in liver cancer and to determine its significance.Methods The expression of KLK9 in liver cancer was detected by immunohistochemistry and RT-PCR techniques.Results The rate of expression of KLK9 protein in liver cancer tissues was significantly higher than paracarcinoma tissues and normal liver tissues (P ＜ 0.05).The expression of KLK9 mRNA in liver cancer cells was significantly higher than normal liver cells (P ＜ 0.05).The expression of KLK9 was related to metastasis,size of tumors,degree of malignancy and clinical staging of the liver cancer (P ≤ 0.05),but there was no associated with age,HbsAg and sex (P ＞ 0.05).Conclusions KLK9 may play an important role in the occurrence and development of liver cancer.It may be used as a tumor marker and a prognostic factor.It may also provide a theoretical basis for the diagnosis and biological targeted therapy of liver cancer.