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目的 了解狼疮肾炎 (LN)肾组织中化学趋化因子激活正常T细胞表达和分泌因子(RANTES)的表达并探讨其与LN肾脏病理改变和肾功能损害的关系。方法 采用微波免疫组织化学染色 (SP法 )和原位分子杂交技术检测LN肾脏RANTES的表达 ,并进一步分析其与LN活动指数、肾脏病理和功能损害的关系。结果 狼疮肾炎组织中RANTES表达显著增高 ,以WHOⅣ型为显著。RANTES在肾小球和肾小管均有表达 ,但肾小管表达更显著。LN肾组织RANTES表达与肾组织活动指数、肾脏病理改变和肾功能损害显著相关。结论 RANTES可能参与了LN的发病机制 ,肾组织中RANTES的表达可作为反映狼疮肾组织活动病变和进行性肾损害的参考指标。
Objective To investigate the expression of RANTES and chemokine-activated RANTES in lupus nephritis (LN) and its relationship with pathological changes of renal nephropathy and renal dysfunction. Methods The expression of RANTES in LN kidney was detected by microwave immunohistochemical staining (SP method) and in situ hybridization (IHC) technique. The relationship between LN activity index, renal pathology and functional impairment was further analyzed. Results The expression of RANTES in lupus nephritis was significantly higher than that in WHOⅣ. RANTES is expressed in both glomerulus and tubules, but tubules are more pronounced. LN renal tissue RANTES expression and renal tissue activity index, renal pathological changes and renal dysfunction were significantly associated. Conclusion RANTES may be involved in the pathogenesis of LN. The expression of RANTES in renal tissues may be used as a reference index to reflect the pathological changes of renal lupus nephritis and progressive renal damage.