论文部分内容阅读
台盼蓝试验是常规而简易的方法,但在检测中阳性结果细胞中溶质酶90%以上泄漏。MTT[3-(4,5-dimethyl-thiazoyl-z-yi)-2,5-dlphenyltetrazollumbromidej方法可在细胞溶质酶泄漏之前抑制产生甲月赞,可用于研究细胞被破坏以前的功能失调。用MTT方法测大鼠肝细胞原代
The trypan blue test is a routine and easy method, but more than 90% of the solutes are leaked in positive results cells in the assay. The MTT [3- (4,5-dimethyl-thiazoyl-z-yi) -2,5-dlphenyltetrazolumbromidej method inhibits the production of Jan-Chan prior to the cytosolic enzyme leak and can be used to investigate previous dysfunctional cells. Primary rat hepatocytes were assayed by MTT method