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不同剂量~(60)Coγ线、中子及X射线照射正常人血后,以丝裂原PHA、ConA、LPS及PWM诱导血内淋巴细胞体外培养转化实验,研究淋巴细胞亚群的辐射损伤效应。实验用~3H-TdR、~(14)UR及~(14)C-缬氨酸放射性核素标记化合物示踪技术,观察不同丝裂原诱导人血淋巴细胞的增殖分化动态;电离辐射对细胞内DNA、RNA及蛋白质合成的抑制作用,并比较其辐射损伤效应。结果:(1)不同丝裂原诱导的正常人血淋巴细胞增殖分化特性各异,分属不同的淋巴细胞亚群。(2)实验以DNA及蛋白质合成中,放射性同位素参入CPM值为指标,观察到ConA、PHA诱导的T淋巴细胞亚群,其辐射损伤效应大于LPS、PWM诱导的B淋巴细胞。(3)淋巴细胞受中子照射后的损伤效应大于γ射线及X射线照射。(4)不同丝裂原诱导的正常人血淋巴细胞亚群,受照射后其细胞DNA链断裂修复能力愈强,该细胞的辐射敏感性就愈低。
After different doses of ~ (60) Coγ ray, neutron and X-ray were irradiated on normal human blood, the in vitro lymphocyte culture and transformation experiments were induced by mitogen PHA, ConA, LPS and PWM to study the radiation damage effect of lymphocyte subsets . The 3H-TdR, ~ (14) UR and ~ (14) C-valine radionuclide labeling compounds were used to observe the proliferation and differentiation of human lymphocytes induced by different mitogen. Ionizing radiation DNA, RNA and protein synthesis in the inhibition, and compare the radiation damage effect. Results: (1) The proliferation and differentiation of normal human lymphocytes induced by different mitogen origins were different and belong to different lymphocyte subsets. (2) In the experiment, the DNA and protein synthesis, radioactive isotope incorporation CPM value as an indicator, ConA, PHA-induced T lymphocyte subsets, the radiation damage effect than LPS, PWM-induced B lymphocytes. (3) The damage effect of lymphocytes irradiated by neutron is greater than γ-ray and X-ray irradiation. (4) The normal human blood lymphocyte subpopulation induced by different mitogen showed that the stronger the ability of cell DNA strand break repair after irradiation, the lower the radiosensitivity of the cell.