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目的探讨脊髓星形胶质细胞和小胶质细胞以及前炎性细胞因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子α(TNFα)在CP/CPPS发生发展中的作用及机制。方法 40只健康雄性Sprague-Dawley(SD)大鼠,随机分为正常不加任何处理的对照组(n=20)、CP/CPPS模型组(n=20),完全福氏佐剂和3%角叉菜胶前列腺内注射造成CP/CPPS模型。通过检测机械性痛阈(PWT)来评价动物痛行为学改变;采用免疫组织化学、逆转录多聚酶链反应(RT-PCR)等方法,检测脊髓星形胶质细胞GFAP和小胶质细胞OX-42的表达以及脊髓前炎性细胞因子IL-1β、IL-6及TNFαmRNA表达的变化,评价脊髓小胶质细胞和星形胶质细胞活化情况及脊髓前炎性细胞因子表达与疼痛行为改变的关系。结果与对照组比较,CP/CPPS模型组大鼠建模后第11天出现PWT明显降低(P<0.05),并呈进行性下降趋势;随着时间进程,免疫组化结果显示模型组动物脊髓小胶质细胞和星形胶质细胞依次活化,小胶质细胞活化开始于建模后第7天,星形胶质细胞活化开始于建模后第14天;模型组动物脊髓IL-1β、IL-6和TNFαmRNA表达较对照组明显增加(P<0.05)。结论在CP/CPPS大鼠中脊髓胶质细胞活化及前炎性细胞因子IL-1β、IL-6和TNFα的表达增加,可能在神经病理性疼痛的产生和维持中起着重要的作用。
Objective To investigate the effects of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor α (TNFα) on the activity of astrocytes and microglia in spinal cord / CPPS occurred in the development of the role and mechanism. Methods Forty healthy male Sprague-Dawley rats were randomly divided into normal control group (n = 20), CP / CPPS model group (n = 20), complete Freund’s adjuvant and 3% Carrageenan intraperitoneal injection resulted in a CP / CPPS model. The animal model of pain was evaluated by detecting the mechanical pain threshold (PWT). Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the changes of GFAP and microglial OX- 42 and the expressions of pro-inflammatory cytokines IL-1β, IL-6 and TNFαmRNA in spinal cord to evaluate the activation of spinal cord microglia and astrocytes and the expression of proinflammatory cytokines and pain behavior in spinal cord relationship. Results Compared with the control group, PWT decreased significantly (P <0.05) on the 11th day in the CP / CPPS model group and decreased progressively. With time course, the immunohistochemistry results showed that the model group animals spinal cord Microglia and astrocyte activation in turn, microglial activation began on the 7th day after modeling, and astrocyte activation started on the 14th day after modeling. In the model group, the levels of IL-1β, Compared with the control group, the expression of IL-6 and TNFα mRNA increased significantly (P <0.05). Conclusion The activation of spinal cord glia and the expression of pro-inflammatory cytokines IL-1β, IL-6 and TNFα in CP / CPPS rats may play an important role in the production and maintenance of neuropathic pain.