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目的探讨p53基因的单核苷酸多态与鼻咽癌(NPC)放疗为主的治疗效果的关系。方法应用PCR-RFLP(聚合酶链反应-限制性片断长度多态性)分析方法,对74例以放疗为主治疗的NPC患者,进行p53第72密码子Arg-Pro多态检测,治疗结束后进行效果评价。以非条件Logistic回归模型比较不同基因型与治疗效果的关系。结果携带Arg/72Pro基因型患者的疗效是携带72Arg/Arg与Pro/Pro基因型患者的2.49倍(95%CI=0.913～6.810,P=0.075);I+II期患者的疗效是III+IV期的3.32倍(95%CI=1.028～10.720,P=0.045);男性患者的疗效是女性的2.39倍(95%CI=0.862～6.670,P=0.094);年龄不是影响敏感性的因素(P=0.194);治疗方式的不同,对疗效未见显著影响。结论与其他两种基因型的携带者相比,携带p53第72密码子Arg/Pro基因型鼻咽癌患者的疗效有可能提高。
Objective To investigate the relationship between the single nucleotide polymorphism of p53 gene and the therapeutic effect of nasopharyngeal carcinoma (NPC) radiotherapy. Methods A total of 74 patients with primary NPC treated by radiotherapy were enrolled in the Arg-Pro polymorphism of p53 site by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) analysis. After treatment, Perform the effect evaluation. The relationship between different genotypes and therapeutic effect was compared with non-conditional Logistic regression model. Results The efficacy of Arg / 72Pro genotype patients was 2.49 times (95% CI = 0.913-6.810, P = 0.075) in patients with 72Arg / Arg and Pro / Pro genotypes. The efficacy of patients with stage I + II disease was III + IV (95% CI = 1.028-10.720, P = 0.045). The effect of male was 2.39 times higher than that of female (95% CI = 0.862-6.670, P = 0.094) = 0.194); different treatment methods, no significant effect on the efficacy. Conclusion The efficacy of Arg / Pro genotype NPC patients with p53 codon 72 codon usage is likely to be enhanced compared with those of the other two genotypes.