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目的 研究 SOD复合酶对兔细菌性角膜溃疡的保护作用及其作用机理。方法 在体外向接种了绿脓杆菌的培养基上分别加入丁胺卡那霉素、SOD复合酶等 ,观察抑菌圈的直径。建立兔眼绿脓杆菌性角膜溃疡模型 ,给予丁胺卡那霉素治疗 ,加用 SOD复合酶作为辅助治疗 ,而灭活 SOD复合酶作为对照 ,观察临床变化 ,并进行病理学检查和丙二醛测定。结果 体外实验证实 SOD复合酶没有抗菌作用。动物实验中 ,加用 SOD复合酶的兔眼 ,临床表现及病理改变较轻 ,丙二醛含量较低。结论 兔眼细菌性角膜溃疡中 ,自由基及脂质过氧化参与了对组织的损伤 ,而 SOD复合酶通过抗自由基及脂质过氧化而消除这种损伤 ,对角膜有一定的保护作用
Objective To study the protective effect of SOD complex enzyme on bacterial corneal ulcer and its mechanism. Methods In vitro to the inoculation of Pseudomonas aeruginosa medium were added amikacin, SOD enzyme complex, observed inhibition zone diameter. To establish a model of P. aeruginosa corneal ulcer in rabbits, give amikacin treatment, add SOD complex enzyme as adjuvant therapy, and inactivate SOD complex enzyme as a control, observe the clinical changes, and pathological examination and two Aldehyde determination. Results In vitro experiments confirmed that SOD complex enzyme has no antibacterial effect. Animal experiments, plus SOD enzyme complex in rabbit eyes, clinical manifestations and pathological changes lighter, malondialdehyde content is lower. Conclusions In bacterial corneal ulcer of rabbits, free radicals and lipid peroxidation are involved in the tissue damage, and SOD complex enzymes eliminate this damage by anti-free radical and lipid peroxidation, which have certain protective effect on the cornea