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目的 探讨线粒体DNA 4977bp缺失突变与皮肤成纤维细胞光老化之间的关系。 方法 采用 8 甲氧补骨脂素及长波紫外线 (8 MOP/UVA)共同作用诱导培养皮肤成纤维细胞光老化 ,一步法提取线粒体DNA(mtDNA) ,三引物PCR的方法检测皮肤成纤维细胞线粒体DNA 4977bp缺失突变 ,密度扫描半定量分析。 结果 8 MOP/UVA作用下 ,培养皮肤成纤维细胞迅速出现细胞老化的形态学改变 ;2 8d时 ,8 MOP/UVA组、UVA组 4977bp缺失突变的发生率及占总mtDNA中的比例分别为 10 0 %、11%和 0 2 5 3、0 0 5 3 ;8 MOP组、正常对照组均为阴性 ,8 MOP/UVA组与其他各组比较差异均有显著性 (P <0 0 1)。 结论 线粒体DNA 4977bp缺失突变累积与皮肤光老化密切相关 ,可能作为衡量皮肤光老化程度的分子生物学标志
Objective To investigate the relationship between mitochondrial DNA 4977 bp deletion mutation and photoaging of skin fibroblasts. Methods 8-methoxy-Psoralen (8-POP) and long-wave ultraviolet (8 MOP / UVA) were used to induce skin fibroblasts to undergo photoaging. Mitochondrial DNA (mtDNA) was extracted by one-step method and mitochondrial DNA 4977bp deletion mutation, semi-quantitative density scanning. Results Under 8 MOP / UVA treatment, the morphological changes of cultured skin fibroblasts rapidly appeared. At 28 days, the incidence of 4977 bp deletion mutation and the proportion of total mtDNA in 8 MOP / UVA group and UVA group were 10 0%, 11%, and 0 2 5 3,0 0 5 3 respectively; all of the 8 MOP group and the normal control group were negative. There was significant difference between 8 MOP / UVA group and other groups (P <0.01). Conclusion The 4977 bp deletion mutation in mitochondrial DNA is closely related to skin photoaging and may be used as a molecular biomarker to measure the degree of skin photoaging