【摘 要】
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We aimed to characterize NDM-5-producing Enterobacteriaceae from aquatic products in Guangzhou,China.A total of 196 intestinal samples of grass carp collected in 2019 were screened for carbapenemase genes.Characterization of blaNDM-5 positive isolates and
【机 构】
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College of Veterinary Medicine,Key Laboratory of Zoonosis of Ministry of Agricultural and Rural Affa
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We aimed to characterize NDM-5-producing Enterobacteriaceae from aquatic products in Guangzhou,China.A total of 196 intestinal samples of grass carp collected in 2019 were screened for carbapenemase genes.Characterization of blaNDM-5 positive isolates and plasmids was determined by antimicrobial susceptibility testing,conjugation experiments,lllumina HiSeq,and Nanopore sequencing.One Citrobacter freundii and six Escherichia coli strains recovered from seven intestinal samples were verified as blaNDM-5 carriers(3.57％,7/196).The b/aNDM-5 genes were located on the lncX3(n=5),lncHI2(n=1),or lncHI2-lncF(n=1)plasmids.All blaNDM-5-bearing plasmids were transferred by conjugation at frequencies of～10-4-10-6.Based on sequence analysis,the lncHI2 plasmid pHNBYF33-1 was similar to other blaNDM-5-carrying lncHI2 plasmids deposited in GenBank from Guangdong ducks.In all lncHI2 plasmids,blaNDM-5 was embedded in a novel transposon,Tn 7051(IS3000-Δ\\SAba125-\\S5-ΔISAba125-blaNDM_5-bleMBL-trpF-tat-Δdct-IS26-ΔumuD-ΔISKox3-\\S3000),which was identical to the genetic structure surrounding blaNDM-5 found in some lncX3 plasmids.The lncHI2-lncF hybrid plasmid pHNTH9F11-1 was formed by homologous recombination of the blaNDM-5-carrying lncHI2 plasmid and a heavy-metal-resistant lncF plasmid through ΔTn 1721.To the best of our knowledge,this is the first report on the characterization of blaNDM-5-bearing plasmids in fish in China.The lncHI2 plasmid pHNBYF33-1 may be transmitted from ducks,considering the common duck-fish freshwater aquaculture system in Guangdong.Tn7051 is likely responsible for the transfer of blaNDM-5 from lncX3 to lncHI2 plasmids in Enterobacteriaceae,resulting in the expansion of transmission vectors of blaNDM-5.
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