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目的研究血红素氧合酶(HO)在门静脉高压症患者脾动脉的表达,探讨血红素氧合酶-内源性一氧化碳系统(HO-CO)在门静脉高压症发病机制中的作用。方法应用半定量逆转录- 聚合酶链反应(RT—PCR)检测HO-1、HO-2 mRNA表达,Western blot进一步检测其蛋白表达。试验组为门脉高压症并行择期脾切除加贲门周围血管离断术患者,同期外伤性脾破裂行脾切除术患者为对照。结果 HO-1mRNA及蛋白仅在门静脉高压症患者组的脾动脉表达,在非门脉高压症患者组不表达,吸光度比值半定量分析结果相比差异都有统计学意义(mRNA 0．81±0．12 vs 0．03± 0．00,P<0．01,蛋白1．56±0．25 vs 0．04±0．01,P<0．01);而HO-2 mRNA及蛋白在门静脉高压症患者组与非门脉高压症患者组都有表达,且吸光度比值半定量分析相比差异均无统计学意义 (mRNA 0．58±0．09 vs 0．64±0．12,P>0．05,蛋白0．92±0．12 vs 0．84±0．14,P>0．05)。结论 HO-CO系统,尤其是HO—1在门静脉高压症发生发展中起重要作用。
Objective To investigate the expression of heme oxygenase (HO) in the splenic artery of patients with portal hypertension and to explore the role of heme oxygenase - endogenous carbon monoxide system (HO-CO) in the pathogenesis of portal hypertension. Methods The mRNA expressions of HO-1 and HO-2 were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and Western blot was used to detect the protein expression. The experimental group was treated with elective splenectomy and peripheral cardioplegia in patients with portal hypertension, and splenectomy with traumatic splenic rupture was performed as control. Results HO-1 mRNA and protein were only expressed in the splenic artery of patients with portal hypertension, but not in patients with non-portal hypertension. There was significant difference in semi-quantitative analysis of HO-1 mRNA and protein expression (mRNA 0.81 ± 0 .12 vs 0.03 ± 0.00, P <0.01, protein 1.56 ± 0.25 vs 0.04 ± 0.01, P <0.01), while HO-2 mRNA and protein in the portal vein Patients with hypertension and non-portal hypertension had expression, and there was no significant difference in the absorbance ratio between the two groups (mRNA 0.58 ± 0.09 vs 0.64 ± 0.12, P> 0.05, protein 0.92 ± 0.12 vs 0.84 ± 0.14, P> 0.05). Conclusion HO-CO system, especially HO-1 plays an important role in the development of portal hypertension.