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免疫毒素杀伤靶细胞的关键取决于抗体对靶细胞的特异性识别和毒素对靶细胞的杀伤效率。本研究比较了我们所研制的两种抗T细胞免疫毒素 (CD5∶rRA和CD5∶Ricin)对靶细胞的特异性杀伤效应。实验结果证实 :①两种免疫毒素在 10 -9- 10 -11mol L浓度范围内均可有效地清除CD5 +T细胞群 ,并对CD2 5 +CD3+激活T细胞具有剂量依赖性抑制作用 ;②两种免疫毒素均对混合淋巴细胞增殖反应有明显抑制作用 ,与CD5∶Ricin比较 ,CD5∶rRA在 10 -10 - 10 -11mol L浓度范围内对T细胞增殖的抑制作用明显减弱 ;③ 10mmol L氯化铵与CD5∶rRA联合应用 ,可增强CD5∶rRA对T细胞的清除效率 ;④两种免疫毒素及氯化铵与CD5∶rRA联合应用在有效杀伤T细胞的浓度范围内 ,对骨髓粒 巨噬系祖细胞的增殖无明显的抑制作用
The key to immunotoxins killing a target cell depends on the specific recognition of the target cell by the antibody and the killing efficiency of the toxin on the target cell. This study compared the specific killing effects of two anti-T cell immunotoxins (CD5: rRA and CD5: Ricin) that we developed on target cells. The results of the experiment confirmed that: (1) Both immunotoxins could effectively eliminate CD5 + T cell population in a concentration range of 10 -9-10 mol·L-1 and dose-dependently inhibit CD25 + CD3 + T cells; Immunotoxins all significantly inhibited the proliferation of mixed lymphocytes. Compared with CD5: Ricin, the inhibitory effect of CD5: rRA on T cell proliferation was significantly weakened in the concentration range of 10 -10 - 10 -11 mol L; The combined application of ammonium chloride and CD5: rRA can enhance the clearance efficiency of CD5: rRA on T cells; ④ The combination of two immunotoxins and ammonium chloride with CD5: rRA can effectively kill T cells, Erythroid progenitor cells proliferation was no significant inhibitory effect