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近年来,随着纳米科技的发展,纳米材料逐渐有了广泛的应用.然而,动物实验和细胞实验的结果表明,许多纳米材料存在潜在的毒性.我们用实验室先前建立的光电化学DNA传感器快速检测了聚苯乙烯纳米球溶液诱导的DNA损伤.在这个传感系统中,我们通过静电组装的方式将双链DNA膜组装到纳米SnO2半导体电极上,然后使用一种DNA双链嵌入剂,即Ru(bpy)2(dppz)2+(bpy=2,2’-bipyridine,dppz=dipyrido[3,2-a:2’,3’-c]phenazine)作为光电信号分子.当电极上的DNA膜暴露于2.0mg/mL的聚苯乙烯纳米球溶液1小时后,电极的光电信号下降了将近20%.光电流的下降是由于DNA膜受到损伤,使得嵌入到电极表面的Ru(bpy)2(dppz)2+信号分子的量减少所导致的.凝胶电泳的实验结果也表明,DNA与聚苯乙烯纳米球溶液孵育后产生了化学损伤.然而,光电化学传感器与凝胶电泳的结果同时还表明,多次清洗后的聚苯乙烯纳米球溶液并不会导致DNA损伤,而未清洗的聚苯乙烯纳米球溶液的上清液却可以导致与聚苯乙烯纳米球溶液大致相当的DNA损伤.清洗后上清液的紫外吸收光谱显示其中含有杂质,且呈现出与氧化苯乙烯类似的特征吸收,而氧化苯乙烯是一种已知的DNA加合损伤试剂.因此,上清液中存在的氧化苯乙烯等杂质可能是导致DNA损伤的主要原因.本研究表明,在进行纳米材料的毒理学研究之前,对于材料的充分表征是非常有必要的.我们报道了光电化学传感器在快速评价纳米材料在DNA损伤上的应用.
In recent years, with the development of nanotechnology, nanomaterials have been widely used.However, the results of animal experiments and cell experiments show that many nanomaterials have potential toxicity.We used the laboratory previously established photoelectrochemical DNA sensor fast We examined the DNA damage induced by polystyrene nanosphere solution.In this sensing system, we assemble the double-stranded DNA membrane onto the nano-SnO2 semiconductor electrode by electrostatic assembly and then use a DNA double-stranded intercalator (Bpy) 2 (dppz) 2+ (bpy = 2,2’-bipyridine, dppz = dipyrido [3,2-a: 2 ’, 3’-c] phenazine) After exposed to 2.0mg / mL polystyrene nanosphere solution for 1 hour, the photoelectric signal of the electrode decreased by nearly 20% .The decrease of photocurrent was due to the damage of the DNA film, so that Ru (bpy) 2 embedded in the electrode surface (dppz) 2+ signal molecules.Experimental results of gel electrophoresis also showed that chemical damage was induced when DNA was incubated with polystyrene nanosphere solution.However, the results of photoelectrochemical sensor and gel electrophoresis Also showed that after repeated washing of polystyrene nanosphere solution It will not lead to DNA damage, but the supernatant of unwashed polystyrene nanosphere solution can lead to DNA damage which is approximately equivalent to that of polystyrene nanosphere solution.Ultraviolet absorption spectrum of supernatant after washing shows that it contains impurities, And exhibits a similar absorption characteristic as styrene oxide, a well-known DNA adduct injury reagent, therefore impurities such as styrene oxide present in the supernatant may be responsible for the DNA damage. This study shows that sufficient characterization of the material is necessary before conducting toxicological studies on nanomaterials.We report the use of photoelectrochemical sensors in the rapid evaluation of nanomaterials for DNA damage.