论文部分内容阅读
采用振镜与特殊设计的f-theta物镜构成光学扫描结构,设计出新型的可用于48通道的毛细管阵列电泳的共焦激光诱导荧光检测系统。根据新型系统的性能需求,分析其设计要求,确定系统结构布局,指导f-theta物镜的设计,并通过软件优化确定最终参数。利用理论分析与光学仿真软件,分析了振镜的离焦对系统荧光收集效率的影响,并定义了荧光收集效率不均匀度,用于度量系统在整个视场中荧光收集效率的非一致性。分析结果表明:离焦将造成荧光收集效率下降,并恶化收集效率的均匀度。最后,通过实验检验系统的荧光收集效率不均匀度。实验结果表明:实际系统满足设计要求。
A novel confocal laser-induced fluorescence detection system for 48-channel capillary array electrophoresis was designed by using galvanometer and specially designed f-theta objective lens to construct the optical scanning structure. According to the performance requirements of the new system, the design requirements are analyzed, the system structure layout is determined, the design of the f-theta objective lens is guided, and the final parameters are determined through software optimization. The theoretical analysis and optical simulation software were used to analyze the influence of galvanometer defocus on the system fluorescence collection efficiency. The definition of the non-uniform fluorescence collection efficiency was used to measure the non-uniformity of the fluorescence collection efficiency in the entire field of view. The results of the analysis show that defocusing causes a decrease in fluorescence collection efficiency and worsens the uniformity of collection efficiency. Finally, the system is experimentally tested for fluorescence nonuniformity of fluorescence collection efficiency. The experimental results show that the actual system meets the design requirements.