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目的探讨具有特定组成的丹酚酸(SA)对四氯化碳(CC l4)所致大鼠肝损伤的预防性保护作用及其机制。方法将健康SD成年大鼠随机分为5组,对照组和模型组给予蒸馏水,各实验组分别用3种不同剂量SA灌胃,2周后以花生油(对照组)或CC l4(其余各组)腹腔注射,24 h后测定大鼠血清天门冬氨酸氨基转移酶(AST)及丙氨酸氨基转移酶(ALT)、血清和肝组织丙二醛(MDA)、谷胱甘肽(GSH)含量以及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性,并观察肝组织病理变化。结果模型组大鼠血清AST、ALT值分别为615 U/L和243 U/L,而SA组大鼠AST、ALT分别为348~452和134~181 U/L,活性显著下降(P<0.05)。与模型组比较,当SA剂量为300mg/(kg.bw)时,大鼠血清和肝匀浆中的SOD活性分别上升22.7%和16.7%,MDA含量降低19.3%和30.5%,GSH含量升高9.2%和9.1%,差异均有统计学意义(P<0.05)。实验组和对照组间GSH-Px活性没有显著变化(P>0.05)。模型组大鼠肝脏小叶中心部出现明显点状和灶状坏死,而SA不同剂量组大鼠肝脏病变明显减轻。结论前期给予适量的SA处理可有效抵抗CC l4所致大鼠急性肝损伤,其保护作用机制可能与提高大鼠机体抗氧化能力有关。
Objective To investigate the protective effect and mechanism of salvianolic acid (SA) with specific composition on hepatic injury induced by carbon tetrachloride (CC14) in rats. Methods Healthy SD adult rats were randomly divided into 5 groups. The control group and model group were given distilled water. Each experimental group was given gavage with 3 different doses of SA. After 2 weeks, the rats were treated with peanut oil (control group) or CC l4 ) Were injected intraperitoneally. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), malondialdehyde (MDA), glutathione (GSH) Content and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and observe the pathological changes of liver tissue. Results The serum AST and ALT values in the model group were 615 U / L and 243 U / L, respectively, while the levels of AST and ALT in SA group were 348 ~ 452 and 134 ~ 181 U / L, respectively ). Compared with model group, SOD activity in serum and liver homogenate increased by 22.7% and 16.7%, MDA content decreased by 19.3% and 30.5%, GSH content increased when SA dose was 300mg / (kg.bw) 9.2% and 9.1% respectively, the difference was statistically significant (P <0.05). There was no significant change in GSH-Px activity between the experimental group and the control group (P> 0.05). In the model group, there were obvious punctate and focal necrosis at the center of the hepatic lobule, while the SA lesion in the different doses of SA significantly reduced. Conclusions Pretreatment with appropriate amount of SA can effectively prevent acute liver injury induced by CC14 in rats, and its protective mechanism may be related to its antioxidant capacity.