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目的应用气管滴注法建立细颗粒物(PM2.5)染尘实验动物模型,探讨细颗粒物对大鼠肺组织的氧化性损伤作用及维生素E在细颗粒物(PM2.5)致肺组织损伤中的拮抗作用。方法健康成年Wistar大鼠48只随机分为空白对照组、生理盐水对照组、低剂量染尘组(1.5 mg/kg·bw)、低剂量染尘干预组、中剂量染尘组(7.5 mg/kg·bw),中剂量染尘干预组、高剂量染尘组(37.5 mg/kg·bw)、高剂量染尘干预组。生理盐水对照组大鼠气管滴注生理盐水(1.5 ml/kg),各染尘组及干预组用各组设定浓度的细颗粒物生理盐水混悬液进行气管滴注,3组干预组初次染尘后即给予添加维生素E的饲料进行干预,添加量为500 mg/kg。每周染尘1次,共4次,于最后一次染尘后2 d内处死,测定肺泡灌洗液中乳酸脱氢酶(LDH),酸性磷酸酶(ACP),碱性磷酸酶(AKP),丙二醛(MDA)活力。结果空白对照组和生理盐水对照组各指标差异均无统计学意义(P>0.05),各剂量染毒组与生理盐水对照组相比差异均有统计学意义(P<0.05),且随着染尘剂量增加各指标活性水平升高,呈现剂量-效应关系。维生素E各干预组与同剂量染尘组比较各指标差异均有统计学意义(P<0.05),即干预组与同剂量染尘组指标水平比较明显降低。结论环境细颗粒物对大鼠肺组织可造成一定程度的氧化性损伤作用,维生素E对其造成的损伤具有一定的拮抗作用。
Objective To establish an experimental animal model of dusting of fine particulate matter (PM2.5) by tracheal instillation, to explore the effect of fine particulate matter on the oxidative damage of lung tissue and the damage of vitamin E to lung tissue caused by fine particulate matter (PM2.5) Antagonism. Methods Forty - eight healthy adult Wistar rats were randomly divided into control group, saline control group, low dose of dust group (1.5 mg / kg · bw), low dose of dust group, middle dose of dust group (7.5 mg / kg · bw), middle dose of dust intervention group, high dose of dust group (37.5 mg / kg · bw), high dose of dust intervention group. Rats in the saline control group were given saline (1.5 ml / kg) by tracheal intratracheal intratracheal instillation of fine particles of normal saline suspension of each concentration group and the intervention group. The primary staining of the three intervention groups After the dust was given vitamin E feed intervention, add the amount of 500 mg / kg. Dust was once a week for 4 times and sacrificed within 2 days after the last dyed dust. Lactate dehydrogenase (LDH), acid phosphatase (ACP), alkaline phosphatase (AKP) , Malondialdehyde (MDA) activity. Results There was no significant difference in each index between blank control group and saline control group (P> 0.05). There was a significant difference between each dose group and saline control group (P <0.05) Dust dose increased the activity of each index increased, showing a dose-response relationship. Vitamin E in each intervention group and the same dose of dust group compared with each index were statistically significant (P <0.05), that is, the intervention group and the same dose of dust group significantly lower levels of indicators. Conclusion Environmental fine particles can cause certain degree of oxidative damage to the lung tissue of rats, and vitamin E can antagonize the damage caused by vitamin E.