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目的:研究IL-24在早孕期母胎界面的表达及其对绒毛外滋养细胞系TEV-1体外侵袭能力的影响。方法:免疫组化法检测早孕期绒毛及蜕膜组织中IL-24的表达情况;MTT法检测人重组IL-24蛋白对TEV-1细胞增殖的影响;用Transwell小室体外侵袭实验,分析绒毛外滋养细胞在IL-24作用下的侵袭能力。结果:早孕期绒毛及蜕膜组织均有IL-24的表达,分布于绒毛柱、滋养细胞、间质及血管内;不同浓度的人重组IL-24蛋白对TEV-1细胞增殖无影响;人重组IL-24对TEV-1细胞的侵袭能力有抑制作用,并呈一定的浓度依赖性。结论:IL-24可抑制TEV-1细胞的侵袭能力,因此早孕期母胎界面产生的IL-24可能影响滋养细胞的侵袭过程,在妊娠过程中有重要意义。
OBJECTIVE: To study the expression of IL-24 in maternal-fetal interface of early pregnancy and its effect on in vitro invasion of villous trophoblast cell line TEV-1. Methods: The expression of IL-24 in villus and deciduas of early pregnancy was detected by immunohistochemistry. The effect of human recombinant IL-24 protein on the proliferation of TEV-1 cells was detected by MTT assay. Trophoblast invasion ability under the action of IL-24. Results: The expression of IL-24 in chorionic villi and decidua in early pregnancy were distributed in villus, trophoblast, stroma and blood vessels. Different concentrations of human recombinant IL-24 protein had no effect on the proliferation of TEV-1 cells. Recombinant IL-24 inhibited the invasiveness of TEV-1 cells in a concentration-dependent manner. Conclusion: IL-24 can inhibit the invasion ability of TEV-1 cells. Therefore IL-24 produced by the maternal-fetal interface in early pregnancy may affect the invasion of trophoblasts and is of great significance in pregnancy.