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茶树作为一种叶用植物,具有重要的经济价值。非生物胁迫对茶树的生长发育过程和茶叶的生产影响很大。本研究采用RT-PCR方法从茶树‘龙井43’cDNA中克隆获得1个编码HD-Zip转录因子的基因CsHB1。序列分析显示,CsHB1基因cDNA长为1 380 bp,编码459个氨基酸,含有典型的START结构域。进化分析显示,茶树CsHB1属于HD-Zip家族转录因子的IV亚族。多序列对比发现,CsHB1与其他物种的HD-Zip类蛋白的氨基酸序列具有78.05%的相似性,如葡萄、烟草、芝麻等。对CsHB1转录因子理化性质、亲/疏水性、无序化分析显示,CsHB1转录因子是疏水性蛋白且显偏碱性,不存在无序化区域。空间结构分析显示,CsHB1有3个α-螺旋,多个β-折叠,且有START结构域。利用荧光定量PCR方法分析了CsHB1在高温(38°C)、低温(4°C)、PEG干旱(200 g·L~(-1))、NaCl(200 mmol·L~(-1))处理1、4、8、12 h的表达情况。结果表明,CsHB1基因在不同非生物胁迫处理下均能诱导表达,且表达差异性明显。
Tea tree as a leaf plant, has important economic value. Abiotic stress has a great influence on the process of tea tree growth and tea production. In this study, a gene encoding HD-Zip transcription factor, CsHB1, was cloned by RT-PCR from Camellia sinensis Longjing 43’cDNA. Sequence analysis showed that the CsHB1 cDNA was 1 380 bp in length and encoded 459 amino acids with a typical START domain. Phylogenetic analysis showed that the tea tree CsHB1 belongs to the subgroup IV of the HD-Zip family of transcription factors. Multiple sequence alignment revealed that CsHB1 shared 78.05% similarity with the HD-Zip proteins of other species such as grapes, tobacco and sesame seeds. The physical and chemical properties of CsHB1 transcription factor, pro / hydrophobic, disordered analysis showed that CsHB1 transcription factor is a hydrophobic protein and was more alkaline, there is no disorder of the region. Spatial structure analysis revealed that CsHB1 has three α-helices, multiple β-sheets, and a START domain. The effect of CsHB1 on the growth of CsHB1 was studied by fluorescence quantitative PCR at high temperature (38 ° C), low temperature (4 ° C), PEG drought (200 g · L -1) and NaCl (200 mmol·L -1) 1,4,8,12 h expression. The results showed that CsHB1 gene can be induced under different abiotic stress conditions and the expression was significant.