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应用套式PCR方法，对44份与B19微小病毒感染症相关的病人血清标本进行检测，其中5份血清标本和扩增产物合有B19病毒的104bpDNA生段，根据B19病毒基因组DNA核苷酸序列分析资料，使用HaeⅢ限制性内切酶切PCR扩增产物，结果产生81和23bp两段DNA，与序列分析结果一致，证实5例患者为B19病毒感染者，从病原学上支持了不久前我们使用血清学方法发现福建省B19病毒感染的报道。
Serological samples of 44 patients with B19 parvovirus infection were detected by nested PCR. Among them, 5 serum samples and amplification products were combined with a 104 bp DNA fragment of B19 virus. Based on the nucleotide sequence of B19 virus genomic DNA, Analysis of the data, the use of Hae Ⅲ restriction endonuclease PCR amplification of the product, resulting in two 81 and 23bp DNA, consistent with the sequence analysis confirmed that five patients with B19 virus infection, echogenic support from the recent Using serological methods to detect B19 virus infection in Fujian Province.