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目的研究大鼠血管狭窄处内皮细胞组织因子(tissue factor,TF)的表达规律。方法将45只SD大鼠(重量320±18.5 g)随机分为对照组和颈动脉狭窄组,狭窄组又分为0.25、0.5、1、2、4、8、16、24 h,8个时相,套扎法建立左颈总动脉狭窄模型,应用数字减影血管造影和多普勒血流仪在体测定切应力及血流量。术后不同时相点用原位杂交和免疫组化法,检测TF的mRNA和蛋白表达。应用图像分析系统,测定内膜平均灰度,进行统计学分析。结果正常对照组内皮细胞TF的mRNA转录和蛋白合成微弱;狭窄15 min后,内皮细胞胞浆TF基因mRNA转录和蛋白合成升高,与对照组比较均有显著性差异(P<0.01)。TF基因mRNA转录和蛋白合成,4 h达到峰值,与邻近组比较具有显著性差异(P<0.01)。结论血管狭窄后,切应力能够早期诱导动脉狭窄处内皮细胞TF基因表达。
Objective To study the expression of endothelial cell tissue factor (TF) in vascular stenosis of rats. Methods 45 SD rats weighing 320 ± 18.5 g were randomly divided into control group and carotid artery stenosis group. The stenosis group was divided into 0.25, 0.5, 1, 2, 4, 8, 16, 24 h and 8 Phase and ligation of the left common carotid artery stenosis model, digital subtraction angiography and Doppler flowmeter in vivo measurement of shear stress and blood flow. The mRNA and protein expression of TF were detected by in situ hybridization and immunohistochemistry at different time points after operation. Application of image analysis system, measuring the average gray level of the endometrium for statistical analysis. Results The mRNA and protein synthesis of TF in normal control group were weak. After 15 min of stenosis, mRNA transcription and protein synthesis of TF gene in endothelial cells increased, which were significantly different from those in control group (P <0.01). TF mRNA transcription and protein synthesis peaked at 4 h, which was significantly different from the adjacent group (P <0.01). Conclusions After vascular stenosis, shear stress can induce endothelial cell TF gene expression in arterial stenosis early.