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目的观察蛋白酶体抑制剂硼替佐米(BTZ)联合顺铂(CIS)对宫颈癌HeLa细胞增殖的影响并研究其相关机制。方法将Hela细胞分为NC组、CIS组、BTZ组、C&B组,分别以DMEM培养液、含CIS的培养液、含BTZ的培养液、含CIS及BTZ的培养液培养。以CCK-8法检测各组细胞的增殖抑制率,Western blotting法检测各组细胞中p53与Rb蛋白的表达水平,流式细胞仪检测细胞凋亡率。组间比较采用析因设计资料的方差分析。结果 C&B组细胞增殖抑制率高于其他各组(P<0.05)。CIS组及BTZ组细胞生长状态比NC组差;C&B组细胞生长状态最差。CIS组及C&B组中p53表达量较高(P<0.05),BTZ组与NC组相比差异无统计学意义。与NC组相比,BTZ组Rb表达量较高(P<0.05),CIS组、C&B组与NC组相比,差异无统计学意义。流式结果示CIS和BTZ在诱导细胞凋亡方面具有交互作用。结论 CIS可能通过调控p53通路对宫颈癌HeLa细胞增殖产生抑制作用,而BTZ可能通过调控Rb通路对宫颈癌HeLa细胞增殖产生抑制作用,且BTZ与CIS联用在抑制HeLa细胞增殖方面具有协同交互作用。
Objective To investigate the effect of proteasome inhibitor bortezomib (BTZ) combined with cisplatin (CIS) on the proliferation of cervical cancer HeLa cells and to study its mechanism. Methods Hela cells were divided into NC group, CIS group, BTZ group and C & amp; B group and cultured in DMEM medium, CIS medium, BTZ-containing medium and CIS and BTZ medium respectively. The inhibitory rate of proliferation of each group was detected by CCK-8 method. The expression of p53 and Rb protein in each group was detected by Western blotting, and the apoptosis rate was detected by flow cytometry. Analysis of variance between groups using factorial design data. Results The inhibitory rate of cell proliferation in C & amp; B group was higher than that in other groups (P & lt; 0.05). The cell growth status in CIS group and BTZ group was worse than that in NC group; the cell growth state in C & amp; B group was the worst. The expression of p53 in CIS group and C & amp; B group was higher than that in NC group (P <0.05). There was no significant difference between BTZ group and NC group. Compared with NC group, the expression of Rb in BTZ group was higher (P <0.05), and there was no significant difference between CIS group, C & amp; B group and NC group. Flow cytometry showed that CIS and BTZ had an interaction in inducing apoptosis. Conclusion CIS may inhibit the proliferation of cervical cancer HeLa cells by regulating the p53 pathway. However, BTZ may inhibit the proliferation of cervical cancer HeLa cells by regulating the Rb pathway, and synergistic interaction may be found between BTZ and CIS in inhibiting HeLa cell proliferation .