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目的:探讨骨质疏松大鼠的脂肪基质细胞有无成骨能力。方法:采用去势法构建SD大鼠骨质疏松模型,设置2组样本,分别为19月龄骨质疏松SD雌性大鼠,正常19月龄SD雌性大鼠。每组大鼠分别取腹股沟脂肪垫中脂肪,用胶原酶消化法培养获得脂肪间充质干细胞,绘制并分析细胞生长曲线;再用经典的骨向诱导液诱导2组ASCs,比较2组细胞骨向分化能力有无差别。结果:骨质疏松大鼠与正常老龄大鼠的ASCs骨向分化能力无明显差别。结论:可用去势法构建SD大鼠骨质疏松模型,并可取其细胞行进一步研究。老龄骨质疏松大鼠的ASCs也可以考虑作为种子细胞促进其自体骨愈合,从而为利用自体ASCs促进骨质疏松骨愈合打下一定的理论基础。
Objective: To investigate the osteogenic capacity of adipose-derived stromal cells in osteoporosis rats. Methods: Osteoporosis model was established by castration in SD rats. Two groups of SD rats were randomly divided into SD female rats of 19 months osteoporosis and SD female rats of 19 months old. Adipose-derived adipose tissue was obtained from adipose tissue of inguinal fat pad in each group. Adipose-derived mesenchymal stem cells were obtained by digestion with collagenase and the cell growth curve was drawn and analyzed. Two groups of ASCs were induced by classical bone inducing fluid. Is there any difference in differentiation ability? Results: The osteogenic differentiation of ASCs in osteoporosis rats and normal aging rats showed no significant difference. Conclusion: Osteoporosis model of SD rats can be constructed by castration method, and its cells can be further studied. ASCs of aged osteoporosis rats can also be considered as seed cells to promote their own bone healing, which will lay a theoretical foundation for the use of ASCs to promote bone healing of osteoporosis.