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采用免疫组织化学的卵白素-生物素-HRP复合物(ABC)法,以二氨基联苯胺(DAB)为呈色剂,观察高亲和性的神经营养物质受体(酪氨酸激酶受体),即TrkA, TrkB和TrkC在大鼠Scapa神经节(前庭神经节,VG)中的分布。结果显示,许多VG神经元分别对3种Trk受体呈阳性反应,受体位于神经元胞体。每一种Trk受体的反应强度在不同神经元间有区别,有弱、中等和强反应之分。对同一种Trk受体而言,阳性神经元的大小不等;统计结果显示:TrkA, TrkB和TrkC阳性神经元平均面积分别为330.8±7.6, 303.89±10.6和355.05±8.3μm2。该研究结果为Trk受体在VG内发挥维持神经元存活、保持其形态特征等的作用提供了形态学支持。
Immunohistochemical avidin-biotin-HRP complex (ABC) method, with diaminobenzidine (DAB) as a colorimetric agent, observed high affinity neurotrophic receptor (tyrosine kinase receptor ), Ie the distribution of TrkA, TrkB and TrkC in the rat Scapa ganglion (vestibular ganglia, VG). The results showed that many VG neurons were positive for each of the three Trk receptors, located in the neuronal soma. The response of each Trk receptor varies among neurons, with weak, moderate, and strong responses. For the same Trk receptor, the positive neurons ranged in size; the statistical results showed that the average area of TrkA, TrkB and TrkC positive neurons were 330.8 ± 7.6, 303.89 ± 10.6 and 355.05 ± 8.3μm2, respectively. The findings provide morphological support for the role of the Trk receptor in maintaining the survival of neurons within VG, maintaining its morphological features, and the like.